Fri, 12/06/2013
Customer is a plant pathologist, and asks about using two MiSeq runs (300bpX2) for sequencing a fungal genome (40-60Mb in size) for a grant proposal.
Fri, 12/06/2013 at 12:01 PM
AccuraScience LB: Your design of having 1 run with pair-ended library and another run with mate-pair library (4-5Kb insert size) is reasonable, but you might want to consider lowering the read length: 300 nt is allowed by MiSeq but the error rate might be too high towards the 3' end of the reads.
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Note: LB stands for Lead Bioinformatician. An AccuraScience LB is a senior bioinformatics expert and leader of an AccuraScience data analysis team.
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