Fri, 01/17/2014
Fri, 01/17/2014 at 9:49 AM
Customer: I was wondering if you provide bioinformatics training as I am very interested to carry out the analysis myself. It is a whole-genome sequencing dataset acquired from an Illumina Miseq sequencer.
Fri, 01/17/2014 at 10:25 AM
AccuraScience LB: We will try to accommodate your interest in learning to do this work - it is one of our objectives to spread our skills to the scientific community thus and make NGS more accessible to everyone through our operation.
One of the first questions I have is, do you happen to have access to a Linux machine with larger memory (say, >=64GB RAM)? A machine with even larger RAM might be needed for doing this work efficiently, but 64GB RAM might be adequate to get the work started.
One way of doing this is for us to complete the work in our own hands first, then we will write down the procedure through which we have done it, and notes of caution for the tricky parts, then you could try to duplicate the work on your own - and we will answer your questions/explain things to you along the way.
There are alternative ways for you to learn NGS data analysis, e.g., http://www.bioinformatics.org/wiki/BI231_NGS_Data_Analysis - though it doesn't seem to cover genome assembly... If you would want this work done and get yourself to know how it is done at the same time, maybe it would be better for you to do it with us.
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Note: LB stands for Lead Bioinformatician. An AccuraScience LB is a senior bioinformatics expert and leader of an AccuraScience data analysis team.
Disclaimer: This text was selected and edited based on genuine communications that took place between a customer and AccuraScience data analysis team at specified dates and times. The editing was made to protect the customer’s privacy and for brevity. The edited text may or may not have been reviewed and approved by the customer. AccuraScience is solely responsible for the accuracy of the information reflected in this text.